Employee Participation in Pollution Reduction: Preliminary Analysis of the Toxics Release Inventory

Can the amount of toxic waste released into the environment by manufacturing facilities be reduced by formally involving employees in pollution prevention? Pursuant to the Pollution Prevention Act of 1990, the U.S. Environmental Protection Agency began requiring that manufacturers report human resource management strategies related to source reduction (reduction of waste at the source), as part of the Toxics Release Inventory (TRI). These strategies often involve employee participation in some form. Here we report the preliminary findings of an ongoing study of the effect of employee participation on source reduction, based on the 1991-1992 TRI database. We find, for example, that manufacturers using a certain combination of three formal employee participation practices had triple the reduction in emissions of manufacturers using none of these practices. We also discuss competing predictors of source reduction, and future research directions

Random record processes and state dependent thinning

AbstractSuppose that a point process N̄t = T1, T2, … if [0, ∞) is thinned by independently retaining Tn with probability pn. Our main examples are the classical p-thinning (pn ≡ p) and the random record process (pn = 1n). When N̄t is a mixed, nonhomogeneous Poisson process, we find conditions under which the thinned process is Poisson. When N̄t is a pure birth process (gamma-mixed Poisson with exponential rate), we show that the record process is Markov renewal, with an interesting structure, and we compare this with related asymptotic results. When N̄t is a Mittag-Leffler renewal process (the homogeneous Poisson is a special case), we give a “Deheuvels-type” representation of the record process (Deheuvels, 1982) and related characterization results

A bacterial artificial chromosome library for the Australian saltwater crocodile (Crocodylus porosus) and its utilization in gene isolation and genome characterization

Background Crocodilians (Order Crocodylia) are an ancient vertebrate group of tremendous ecological, social, and evolutionary importance. They are the only extant reptilian members of Archosauria, a monophyletic group that also includes birds, dinosaurs, and pterosaurs. Consequently, crocodilian genomes represent a gateway through which the molecular evolution of avian lineages can be explored. To facilitate comparative genomics within Crocodylia and between crocodilians and other archosaurs, we have constructed a bacterial artificial chromosome (BAC) library for the Australian saltwater crocodile, Crocodylus porosus. This is the first BAC library for a crocodile and only the second BAC resource for a crocodilian. Results The C. porosus BAC library consists of 101,760 individually archived clones stored in 384-well microtiter plates. Not I digestion of random clones indicates an average insert size of 102 kb. Based on a genome size estimate of 2778 Mb, the library affords 3.7 fold (3.7×) coverage of the C. porosus genome. To investigate the utility of the library in studying sequence distribution, probes derived from CR1a and CR1b, two crocodilian CR1-like retrotransposon subfamilies, were hybridized to C. porosusmacroarrays. The results indicate that there are a minimum of 20,000 CR1a/b elements in C. porosus and that their distribution throughout the genome is decidedly non-random. To demonstrate the utility of the library in gene isolation, we probed the C. porosus macroarrays with an overgo designed from a C-mos (oocyte maturation factor) partial cDNA. A BAC containing C-mos was identified and the C-mos locus was sequenced. Nucleotide and amino acid sequence alignment of the C. porosus C-mos coding sequence with avian and reptilian C-mos orthologs reveals greater sequence similarity between C. porosus and birds (specifically chicken and zebra finch) than between C. porosus and squamates (green anole). Conclusion We have demonstrated the utility of the Crocodylus porosus BAC library as a tool in genomics research. The BAC library should expedite complete genome sequencing of C. porosus and facilitate detailed analysis of genome evolution within Crocodylia and between crocodilians and diverse amniote lineages including birds, mammals, and other non-avian reptiles

Estimation of viral richness from shotgun metagenomes using a frequency count approach

BACKGROUND: Viruses are important drivers of ecosystem functions, yet little is known about the vast majority of viruses. Viral shotgun metagenomics enables the investigation of broad ecological questions in phage communities. One ecological characteristic is species richness, which is the number of different species in a community. Viruses do not have a phylogenetic marker analogous to the bacterial 16S rRNA gene with which to estimate richness, and so contig spectra are employed to measure the number of virus taxa in a given community. A contig spectrum is generated from a viral shotgun metagenome by assembling the random sequence reads into groups of sequences that overlap (contigs) and counting the number of sequences that group within each contig. Current tools available to analyze contig spectra to estimate phage richness are limited by relying on rank-abundance data. RESULTS: We present statistical estimates of virus richness from contig spectra. The program CatchAll (http://www.northeastern.edu/catchall/) was used to analyze contig spectra in terms of frequency count data rather than rank-abundance, thus enabling formal statistical analyses. Also, the influence of potentially spurious low-frequency counts on richness estimates was minimized by two methods, empirical and statistical. The results show greater estimates of viral richness than previous calculations in nearly all environments analyzed, including swine feces and reclaimed fresh water. CONCLUSIONS: CatchAll yielded consistent estimates of richness across viral metagenomes from the same or similar environments. Additionally, analysis of pooled viral metagenomes from different environments via mixed contig spectra resulted in greater richness estimates than those of the component metagenomes. Using CatchAll to analyze contig spectra will improve estimations of richness from viral shotgun metagenomes, particularly from large datasets, by providing statistical measures of richness

Hotspot motion caused the Hawaiian-Emperor Bend and LLSVPs are not fixed

Controversy surrounds the fixity of both hotspots and large low shear velocity provinces (LLSVPs). Paleomagnetism, plate-circuit analyses, sediment facies, geodynamic modeling, and geochemistry suggest motion of the Hawaiian plume in Earth's mantle during formation of the Emperor seamounts. Herein, we report new paleomagnetic data from the Hawaiian chain (Midway Atoll) that indicate the Hawaiian plume arrived at its current latitude by 28 Ma. A dramatic decrease in distance between Hawaiian-Emperor and Louisville chain seamounts between 63 and 52 Ma confirms a high rate of southward Hawaiian hotspot drift (similar to 47 mm yr(-1)), and excludes true polar wander as a relevant factor. These findings further indicate that the Hawaiian-Emperor chain bend morphology was caused by hotspot motion, not plate motion. Rapid plume motion was likely produced by ridge-plume interaction and deeper influence of the Pacific LLSVP. When compared to plate circuit predictions, the Midway data suggest similar to 13 mm yr(-1) of African LLSVP motion since the Oligocene. LLSVP upwellings are not fixed, but also wander as they attract plumes and are shaped by deep mantle convection